1. 定义: 'cytof'是一个缩写词,它代表一种分析单个细胞中多个蛋白质分子的技术,全称是cytometry by time-of-flight。
例句:
- Recent advances in single-cell technology, such as cytof, have enabled the study of cellular heterogeneity at unprecedented resolution.(最近单细胞技术的进展,如cytof,使得研究细胞异质性的分辨率前所未有。)
- The cytof data revealed unexpected cell populations that were not detectable by conventional flow cytometry. (cytof数据揭示了不同寻常的细胞群体,这些群体通过常规流式细胞技术是不可检测的。)
2. 原理:cytof利用mass cytometry技术,将单个细胞中的多种细胞因子标记上不同的金属离子,然后通过时间飞行质谱法(time-of-flight mass spectrometry)来分析和鉴定每个细胞中的这些标记。
例句:
- The principle of cytof is to replace fluorochromes with heavy metal isotopes, which can be uniquely identified by mass spectrometry. (cytof的原理是用重金属同位素代替荧光染料,可以通过质谱法唯一鉴定。)
- Cytof can detect up to 50 markers per cell, which allows for high-dimensional characterization of cell phenotypes. (cytof可以检测每个细胞50个标记,允许对细胞表型进行高维度的特征描述。)
3. 应用:cytof广泛用于研究单个细胞中的细胞因子信号通路、生物发育、免疫学和肿瘤学等领域。
例句:
- Cytof has been instrumental in identifying novel cell types and signaling pathways involved in cancer progression.(cytof在鉴定参与癌症发展的新型细胞类型和信号通路方面发挥了关键作用。)
- The application of cytof has greatly improved our understanding of immune cell activation and differentiation. (cytof的应用极大地提高了我们对免疫细胞激活和分化的理解。)
4. 优点:与传统流式细胞技术相比,cytof具有更高的灵敏度、更广泛的多标记检测和更低的自发荧光干扰。
例句:
- One of the major advantages of cytof over traditional flow cytometry is its ability to detect rare cell populations with high precision. (cytof相对于传统流式细胞技术的主要优点之一是它可以高精度地检测罕见的细胞群体。)
- The use of cytof has greatly reduced the need for compensation, which is a major bottleneck in flow cytometry analysis. (cytof的使用极大地降低了修正的需求,这是流式细胞分析的一个主要瓶颈。)
5. 局限性:cytof也存在一些限制,例如需要高度纯化的细胞样本、较高的成本和复杂的数据解析过程。
例句:
- The cost of running a cytof experiment can be quite high, which limits its accessibility to some research groups.(运行cytof实验的成本可能相当高,这限制了一些研究团队的接触。)
- The interpretation of cytof data can be challenging, as it requires advanced computational tools and expertise in data analysis. (cytof数据的解释可能具有挑战性,因为它需要先进的计算工具和数据分析专业知识。)
中文翻译:CyTOF(Mass Cytometry,质谱流式细胞术)
读音:[ˈsaɪtɑːf]
例句:CyTOF技术是一种高分辨率、高通量的单细胞分析技术,能够同时分析多达50种或以上的蛋白质标记,可以用于深入研究免疫细胞亚群的异质性和功能。 (CyTOF technology is a high-resolution, high-throughput single-cell analysis technique that can simultaneously analyze up to 50 or more protein markers, and can be used to study the heterogeneity and function of immune cell subpopulations in depth.)
评论列表